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Advantages of ultrasonic cell disrupter and problems that need attention
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Advantages of ultrasonic cell disrupter and problems that need attention

Views:0     Author:Site Editor     Publish Time: 2020-08-10      Origin:Site

During the development of the ultrasonic cell pulverizer, it is characterized by high-activity pulverization of cells, which improves the efficiency of sample processing and the broken rate of bacterial cells. Once the sample is processed, the broken rate of bacterial cells can reach more than 99%. And the physiological activity of the target protein is protected to the greatest extent, and some preliminary results have been achieved.


Ultrasonic cell crusher uses ultra-high pressure energy to break cells. Compared with ultrasonic, ultrasonic cell crusher has the advantages of maintaining cell viability, saving time, less sample loss, and processing a large number of samples. Therefore, the continuous development and improvement of ultrasonic cell pulverizers have made considerable contributions to mass production.

In the biotechnology industry, the cost of separation and purification of ultrasonic cell pulverizers often accounts for 60% to 90% of the cost of the entire process. my country's biotechnology industry is dominated by the traditional fermentation industry. Product separation and purification is the most important stage of the fermentation industry. It is the downstream of the entire fermentation production line. It not only determines the yield, but also determines the quality and safety of the product. This part includes many operations: separation of cells and fermentation broth, broken cells to release intracellular products, membrane separation to remove cell debris, etc.


Ultrasonic cell crusher is the most commonly used crushing method in biological production at present. Fermentation broth of different strains. The effect of ultrasonic disruption is quite different. Generally, bacilli are easier to break than cocci, and gram-negative bacterial cells are easier to break than gram-positive bacterial cells, and the effect on yeast is less. Advantages: simple operation, less liquid loss, suitable for laboratory scale. Disadvantages: high cost; easy to cause a sharp rise in temperature; in large-scale operation, sound energy transmission and heat dissipation are difficult, and the generated chemical radical groups are easy to inactivate the product.


There are many manufacturers that produce ultrasonic cell crushers, and their products are different in technology content, which directly causes the life of the ultrasonic cell crusher to change. How long can the ultrasonic cell crusher be used? Let's follow Shanghai together. Let's take a look at the question of how long the ultrasonic cell crusher can be used.

How long can the ultrasonic cell crusher be used? This is actually a difficult question. How long the ultrasonic cell crusher can be used is related to many factors. It is not only related to the quality of the ultrasonic cell crusher, but also to the operation of the ultrasonic cell crusher. Relationship. Generally speaking, the better the quality of the ultrasonic cell crusher, the longer the corresponding use time, on the contrary, the shorter.


The daily use of the ultrasonic cell crusher will also affect its lifespan. Generally, it will be used for a longer time if it is properly maintained and operated correctly. On the contrary, it will reduce the service life of the ultrasonic cell crusher, so we are using ultrasonic cells During the process of the crusher, you must read the manual before using it, so that the ultrasonic cell crusher we bought can last longer.


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